UV –Visible Spectroscopy Method Development and Its Validation for the Analysis of Marketed Hair Dyes for Amine Content

To develop a simple rapid, accurate and spectrophotometric reproducible method were developed for estimation of PARA - PHENYLENE DIAMINE in different Marketed Hair dyes . The analysis PPD was performed using NaOH solution as diluent folins reagent at 432nm respectively .The methods linear the concentration range from 0-50µg/ml. validated with respect to system suitability, linearity, precision, limit detection, quantification, accuracy, ruggedness robustness can be used routine marketed hair products. accordance ICH guidelines.